Poster
NanoAmes — Mutagenicity Testing with Micro-Quantities
Pharma Cosmetic +1
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Poster
In Vitro Pig-a Mutation Test on L5178Y Cells
Developing an in vitro Pig-a gene mutation assay on L5178Y cells — a mammalian-cell alternative for evaluating the mutagenic potential of pharmaceuticals and cosmetic ingredients.
What's inside
- The Pig-a gene mutation endpoint (PIG-A)
- In vitro development on L5178Y cells
- A mammalian-cell complement to the Ames test
- Application to pharmaceuticals and cosmetic ingredients
Evaluating the mutagenic potential of compounds remains a key issue for the pharmaceutical, cosmetic and chemical industries. The Ames test remains the gold-standard first-line screen, and the Mouse Lymphoma Assay (MLA) is a well-known mammalian-cell test — but the MLA shows limits, with a percentage of false positives.
The Pig-a assay, originally developed in vivo, detects mutation at the Phosphatidyl-Inositol-Glycan class A (PIG-A) gene. This work develops an in vitro Pig-a test on L5178Y cells, offering a sensitive mammalian-cell complement to the Ames test for pharmaceuticals and cosmetic ingredients.
Who this is for
- Pharmaceutical genotoxicity teams
- Cosmetic ingredient safety
- Academic toxicology
Recommended citation
GenEvolutioN Scientific Team (2024). In Vitro Pig-a Mutation Test on L5178Y Cells. GenEvolutioN Technologies.