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Poster
Dicentric Assay with Telomere & Centromere FISH
How telomere and centromere FISH (PNA) staining improves chromosomal aberration detection in the dicentric and micronucleus assays — applied to biological dosimetry and the RENEB 2021 inter-laboratory exercise.
What's inside
- Dicentric chromosome assay (DCA) for biological dosimetry
- Cytokinesis-block micronucleus assay (CBMN)
- Telomere & centromere PNA-FISH staining
- RENEB 2021 inter-laboratory comparison
Cytogenetic assays for biological dosimetry — the dicentric chromosome assay (DCA) and the cytokinesis-block micronucleus assay (CBMN) — traditionally require a high level of expertise to score chromosomal aberrations reliably.
This work shows that telomere and centromere PNA-FISH staining markedly improves dicentric and chromosomal-aberration detection, making evaluation more accessible. The approach was validated through participation in the RENEB 2021 inter-laboratory comparison exercise.
Who this is for
- Radiobiology and cytogenetics researchers
- Pharma genotoxicity teams
- Biodosimetry laboratories
Recommended citation
C. Cuceu Petrenci, I. Mouche, F. Finot et al. (GenEvolutioN, CEA Saclay) (2023). Dicentric Assay with Telomere & Centromere FISH. GenEvolutioN Technologies.