Poster
In Vitro Pig-a Mutation Test on L5178Y Cells
Pharma Cosmetic
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Poster
Miniaturized Cell Transformation Assay (CTA)
Miniaturizing the Bhas 42 Cell Transformation Assay to detect non-genotoxic carcinogens with minimal test article — including NIAS — with more specific foci labeling and transcriptomic markers.
What's inside
- Detecting non-genotoxic carcinogens with the CTA
- Bhas 42 cell transformation model
- Miniaturisation for low test-article quantities and NIAS
- Specific foci labeling and transcriptomic markers
The Cell Transformation Assay (CTA) is one of the few in vitro tools able to detect non-genotoxic carcinogens. Using Bhas 42 cells — a 3T3 mouse fibroblast line transfected with the Ras gene — cellular transformation produces foci characterised by deep basophilic staining, spindle-shaped cells and multilayer, randomly oriented growth.
This work miniaturises the classical CTA to work with less test article and within complex mixtures or Non-Intentionally Added Substances (NIAS), establishes more specific and sensitive labeling of transformed foci, and explores transcriptomic markers impacted by non-genotoxic carcinogens.
Who this is for
- Cosmetic safety (NIAS, non-genotoxic carcinogens)
- Chemical and pharmaceutical R&D
- Academic toxicology
Recommended citation
GenEvolutioN Scientific Team (2023). Miniaturized Cell Transformation Assay (CTA). GenEvolutioN Technologies.