Poster
In Vitro Pig-a Mutation Test on L5178Y Cells
Pharma Cosmetic
View resource →
Poster
Semi-Automated Micronucleus Assay with Telomere & Centromere Staining
Re-evaluating the cytokinesis-block micronucleus assay with semi-automated scoring following PNA telomere and centromere staining — a new tool for genotoxic risk assessment.
What's inside
- Cytokinesis-block micronucleus (CBMN) assay re-evaluated
- PNA telomere & centromere staining
- Semi-automated micronucleus scoring
- Improved, higher-throughput genotoxic risk assessment
The cytokinesis-block micronucleus (CBMN) assay is a cornerstone of genotoxic risk assessment, but manual scoring is labour-intensive and expertise-dependent.
This work re-evaluates the CBMN assay using semi-automated scoring following PNA telomere and centromere staining. The approach improves the reliability and throughput of micronucleus scoring and adds mechanistic insight (aneugenic vs clastogenic events), providing a new tool for genotoxic risk assessment.
Who this is for
- Cytogenetics and radiobiology researchers
- Pharma genotoxicity teams
- Academic toxicology
Recommended citation
F. Finot, R. M'kacher et al. (multi-centre collaboration) (2023). Semi-Automated Micronucleus Assay with Telomere & Centromere Staining. GenEvolutioN Technologies.